Table of Contents (How to prepare PRP?)
- How to prepare PRP by using IPPOCARE Classic PRP tube – Procedure
- Intended Use
- Product Description
- Storage
- Caution
HOW TO PREPARE PRP – PROCEDURE
How to prepare PRP? Step 1 – DRAW BLOOD
Remove the IPPOCARE Classic PRP tube from the bag. Wipe top of PRP tube with a clean alcohol swab prior to blood draw. Following accepted blood collection guidelines, draw the patient’s blood into PRP tube from a peripheral location.
How to prepare PRP? Step 2 – INVERT TUBE
• Invert PRP tube(s) 7 (seven) times to mix ACD-A anti-coagulant with the patients’ blood.
• Wipe the top of the tube with a clean alcohol swab.
• PRP tube to be processed within six (6) minutes after blood draw.
How to prepare PRP? Step 3 – SPIN PRP TUBE IN CENTRIFUGE
SETTINGS: TEN(10)MINUTES; SPEED 1900 RCF
Power on centrifuge and open up the lid. Place PRP tube(s) into individual tube holders in order to achieve a balanced load within the centrifuge. Place tubes across from each other or use counterbalance, as appropriate. Close the lid.
IMPORTANT NOTE!
Please refer to the centrifuge Instructions for Use for proper usage guidelines. Different sizes of centrifuges operating at the same speed may yield varying results. Therefore, we recommend using a speed of 1900 RCF for 10 minutes to achieve optimal results.
CAUTION: If you have your own Centrifuge, please follow manufacturer’s Instructions for Use for the centrifuge.
a) Centrifuge carriers and inserts should be of the size specific for the tube used.
b) Always allow the centrifuge to come to a complete stop before removing tubes.
c) Counterbalance tubes will be required to balance the weight of the tubes during centrifugation. When using a counterbalance tube, place the balance tube with the same weight as the filled PRP tube, opposite of the PRP tube.
d) Once the centrifugation spin cycle has stopped, open lid of centrifuge and examine tubes for any breakage or leaking prior to use.
How to prepare PRP? Step 4 – PRP EXTRACTION – INVERT TUBE
Remove Classic PRP tube(s) from centrifuge tube holders. Extract PRP or PPP from the tube depending on your needs and injected to treated areas.
CAUTION: Do not insert the needle into the separating gel. If needle comes in contact with the separating gel, discard the product.
• Alcohol swab the top of the PRP tube.
• Invert PRP tube
• Insert sterile syringe to a long needle (e.g. 18Ga x 1.5 inch)
• Insert long needle through the stopper
• Aspirate the PRP into the syringe.
• Once the PRP is transferred into the syringe, the product is ready for use.
INTENTION OF USE
IPPOCARE Classic PRP Tube is designed to be used for the safe and rapid preparation of autologous platelet-rich plasma (PRP) from a small sample of peripheral blood at the patient point-of care. The PRP is mixed with autograft and/or allograft bone prior to application to a bony defect for improving handling characteristics.
PRODUCT DESCRIPTION
Our IPPOCARE Classic PRP Tubes is provided sterile and non-pyrogenic, utilizes a separator gel to isolate the platelets while eliminating the undesirable components such as red blood cells and inflammatory white blood cells. IPPOCARE Platelet-Rich Plasma Tubes allows you to remove red cells, white cells and platelet poor plasma (PPP) without removing a high percentage of platelets. With higher concentrations you can deliver a higher number of total platelets in fewer injections.
NOTE:
• For Single Patient Use Only. Once used, the blood collection tube must be discarded following the protocol for biohazard waste disposal, as required by the facility of use.
• The blood collection tube must be processed via centrifuge within 6 (six) minutes following blood draw.
• The PRP must be used within 4 (four) hours after drawing blood.
STORAGE
Store tubes at 4-40°C (39-104°F). Do not freeze.
CAUTION
a) PRP must be used on the same patient from which blood was drawn.
b) Do not use blood collection tube if any foreign matter is visible.
c) Do not use blood collection tubes that are, or appear to be, cracked or damaged.
d) Do not use blood collection tube after expiration date.
e) Do not re-sterilize or attempt to clean the blood collection tubes.
f) The PRP must be used within 4 (four) hours after drawing blood.
g) Practice Universal Precautions to protect from blood spatter and blood leak age to minimize the potential exposure to blood borne pathogens.
h) Handle all biologic samples and blood collection devices per the procedures established by facility of use.
i) Appropriate medical attention is required in the event of any exposure to biologic samples.
j) Under-filled tubes may result in poor product performance. It is recommended to discard an under-filled tube and re-draw blood into a second tube.