This guide walks you through the full process of preparing Platelet-Rich Plasma (PRP) using the IPPOCARE Classic PRP Tube — a triple-sterile, ACD-based tube optimized for aesthetics and hair restoration. Whether you’re a dermatologist, clinic owner, or medical aesthetician, this step-by-step tutorial ensures consistent results and high platelet recovery.
Correct handling and preparation are essential for obtaining a high concentration of platelets with minimal contamination. Poor preparation can reduce the effectiveness of PRP treatments for skin rejuvenation, hair restoration, or orthobiologics.
Remove the IPPOCARE Classic PRP tube from its sterile pouch. Clean the rubber stopper with a sterile alcohol swab. Use a butterfly needle or standard venipuncture technique to draw peripheral blood into the PRP tube.
Tip: Ensure the correct fill volume (approx. 10mL) to maintain proper anticoagulant ratio.
• Immediately after blood collection, Invert PRP tube(s) 7 (seven) times to mix ACD-A anti-coagulant with the patients’ blood.
• Wipe the top of the tube with a clean alcohol swab.
• PRP tube to be processed within six (6) minutes after blood draw.
Why this matters: Proper mixing prevents clotting, ensuring platelet viability.
Common mistake: Shaking instead of gentle inversion. This can cause platelet damage.
Place the filled tubes into a centrifuge. Use a balance tube of equal volume opposite your PRP tube to maintain stability.
IMPORTANT NOTE!
Always refer to the centrifuge’s user manual. Tubes must remain upright, and centrifugation should begin promptly. Different sizes of centrifuges operating at the same speed may yield varying results. Therefore, we recommend using a speed of 1900 RCF for 10 minutes to achieve optimal results.
Pro Tip: Using a PRP-specific centrifuge (like IPPOCARE’s model) improves separation consistency.
a) Centrifuge carriers and inserts should be of the size specific for the tube used.
b) Always allow the centrifuge to come to a complete stop before removing tubes.
c) Counterbalance tubes will be required to balance the weight of the tubes during centrifugation. When using a counterbalance tube, place the balance tube with the same weight as the filled PRP tube, opposite of the PRP tube.
d) Once the centrifugation spin cycle has stopped, open lid of centrifuge and examine tubes for any breakage or leaking prior to use.
After centrifugation, observe the separation:
Use a sterile syringe with a long needle to aspirate the PRP above the gel layer. Extract PRP or PPP from the tube depending on your needs and injected to treated areas.
CAUTION: Do not insert the needle into the separating gel. If needle comes in contact with the separating gel, discard the product.
• Alcohol swab the top of the PRP tube.
• Invert PRP tube
• Insert sterile syringe to a long needle (e.g. 18Ga x 1.5 inch)
• Insert long needle through the stopper
• Aspirate the PRP into the syringe.
• Once the PRP is transferred into the syringe, the product is ready for use.
IPPOCARE Classic PRP Tube is designed to be used for the safe and rapid preparation of autologous platelet-rich plasma (PRP) from a small sample of peripheral blood at the patient point-of care. The PRP is mixed with autograft and/or allograft bone prior to application to a bony defect for improving handling characteristics.
Our IPPOCARE Classic PRP Tubes is provided sterile and non-pyrogenic, utilizes a separator gel to isolate the platelets while eliminating the undesirable components such as red blood cells and inflammatory white blood cells. IPPOCARE Platelet-Rich Plasma Tubes allows you to remove red cells, white cells and platelet poor plasma (PPP) without removing a high percentage of platelets. With higher concentrations you can deliver a higher number of total platelets in fewer injections.
NOTE:
• For Single Patient Use Only. Once used, the blood collection tube must be discarded following the protocol for biohazard waste disposal, as required by the facility of use.
• The blood collection tube must be processed via centrifuge within 6 (six) minutes following blood draw.
• The PRP must be used within 4 (four) hours after drawing blood.
Store tubes between 4°C–40°C (39–104°F)
Do not freeze
Keep in dry, dark place
Shelf Life: 24 months from manufacturing date
a) PRP must be used on the same patient from which blood was drawn.
b) Do not use blood collection tube if any foreign matter is visible.
c) Do not use blood collection tubes that are, or appear to be, cracked or damaged.
d) Do not use blood collection tube after expiration date.
e) Do not re-sterilize or attempt to clean the blood collection tubes.
f) The PRP must be used within 4 (four) hours after drawing blood.
g) Practice Universal Precautions to protect from blood spatter and blood leak age to minimize the potential exposure to blood borne pathogens.
h) Handle all biologic samples and blood collection devices per the procedures established by facility of use.
i) Appropriate medical attention is required in the event of any exposure to biologic samples.
j) Under-filled tubes may result in poor product performance. It is recommended to discard an under-filled tube and re-draw blood into a second tube.
1. Can I use any PRP tube for this method?
No. We recommend using PRP tubes with ACD and separation gel, like the IPPOCARE Classic PRP Tube.
2. Do I need to add calcium chloride?
No. IPPOCARE PRP tubes are activator-free and require no additional CaCl₂.
3. What is the expected PRP yield?
From a 10mL blood draw, you can expect ~6–8mL PRP depending on technique.
4. Can these tubes be used for PRF too?
No. PRF requires different anticoagulant-free tubes.
5. Where can I order more tubes?
Visit our Classic PRP Tube page or our PRP Tube Wholesale page.
IPPOCARE PRP tubes are CE/ISO certified, triple-sterile, and designed for high-yield PRP extraction.
In 2025, Platelet-Rich Plasma (PRP) therapy remains one of the most trusted regenerative treatments in dermatology, aesthetics, and hair restoration. But not all PRP tubes deliver the same quality or consistency. The type of tube you use—its size, additive, and compatibility—can directly impact your platelet recovery, workflow, and treatment outcomes.
Discover the differences between 15ml and 10ml PRP tubes for your clinic. Learn how each size impacts PRP therapy, treatment outcomes, and patient satisfaction. Choose the right PRP tubes for optimal results!
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