Table of Contents (How to prepare PRP?)
- How to prepare PRP by using IPPOCARE PRP Tube + HA (Hyaluronic Acid) – Procedure
- Intended Use
- Product Description
- Storage
- Caution
HOW TO PREPARE PRP – PROCEDURE
How to prepare PRP+HA? Step 1 – DRAW BLOOD
Remove the IPPOCARE PRP Tube + HA (Hyaluronic Acid) from the bag. Wipe top of PRP tube with a clean alcohol swab prior to blood draw. Following accepted blood collection guidelines, draw the patient’s blood into PRP tube from a peripheral location.
How to prepare PRP+HA? Step 2 – INVERT TUBE
• Invert PRP tube(s) 7 (seven) times to mix ACD-A anti-coagulant with the patients’ blood.
• Wipe the top of the tube with a clean alcohol swab.
• PRP+HA tube to be processed within six (6) minutes after blood draw.
How to prepare PRP+HA? Step 3 – SPIN PRP TUBE IN CENTRIFUGE
SETTINGS: TEN(10)MINUTES; SPEED 1900 RCF
Power on centrifuge and open up the lid. Place PRP+HA tube(s) into individual tube holders in order to achieve a balanced load within the centrifuge. Place tubes across from each other or use counterbalance, as appropriate. Close the lid.
IMPORTANT NOTE!
Please refer to the centrifuge Instructions for Use for proper use. Please note that different sizes of centrifuges with the same RPM might yield varying results. Therefore, we recommend using a speed of 1900 RCF for 10 minutes to achieve the best results.
CAUTION: If you have your own Centrifuge, please follow manufacturer’s Instructions for Use for the centrifuge.
a) Centrifuge carriers and inserts should be of the size specific for the tube used.
b) Always allow the centrifuge to come to a complete stop before removing tubes.
c) Counterbalance tubes will be required to balance the weight of the tubes during centrifugation. When using a counterbalance tube, place the balance tube with the same weight as the filled PRP tube, opposite of the PRP tube.
d) Once the centrifugation spin cycle has stopped, open lid of centrifuge and examine tubes for any breakage or leaking prior to use.
How to prepare PRP+HA? Step 4 – PRP EXTRACTION – INVERT TUBE
Remove PRP+HA tube(s) from centrifuge tube holders. Extract PRP or PPP from the tube depending on your needs and injected to treated areas.
After the centrifugation process, you’ll observe that the PRP layer, containing an abundance of platelets and growth factors, has formed atop the separated blood components due to its lighter density. Directly above the PRP layer, the Hyaluronic Acid mixture will be situated.
To create a homogeneous combination of the PRP and Hyaluronic Acid, gently agitate the tube. This subtle shaking motion will facilitate the thorough mixing of the PRP and Hyaluronic Acid, ensuring their seamless integration.
Consistency Check: Following the shaking, visually inspect the mixture to ensure that the PRP and Hyaluronic Acid have amalgamated into a uniform, gel-like substance.
Application: With the PRP and Hyaluronic Acid now harmoniously combined, the resulting mixture is primed for application to the designated area using your chosen method (micro-needling, injection, or topical application).
CAUTION: Do not insert the needle into the separating gel. If needle comes in contact with the separating gel, discard the product.
• Alcohol swab the top of the PRP tube.
• Invert PRP tube
• Insert sterile syringe to a long needle (e.g. 18Ga x 1.5 inch)
• Insert long needle through the stopper
• Aspirate the PRP into the syringe.
• Once the PRP is transferred into the syringe, the product is ready for use.
INTENTION OF USE
The Intention of Use for PRP+HA (Platelet-Rich Plasma + Hyaluronic Acid) tubes is to offer a comprehensive solution for advanced skin rejuvenation and hair restoration treatments. These tubes are intended for medical professionals to harness the combined benefits of PRP’s regenerative properties, containing platelets and growth factors, and the hydrating effects of Hyaluronic Acid. This combination is designed to address facial rejuvenation by targeting fine lines, wrinkles, and skin volume loss, as well as to stimulate hair follicles for increased hair growth and density. PRP+HA treatments also contribute to improved skin texture, collagen production, hydration, and natural-looking results. It’s essential that these tubes are administered by qualified healthcare providers with expertise in aesthetic procedures to ensure safe and effective outcomes tailored to individual needs and aspirations.
PRODUCT DESCRIPTION
The PRP+HA Tube represents a cutting-edge solution that combines the regenerative power of Platelet-Rich Plasma (PRP) with the hydrating benefits of Hyaluronic Acid (HA). This advanced product is meticulously designed for medical professionals aiming to provide optimal skin rejuvenation and hair restoration results. The PRP component, rich in platelets and growth factors, supports tissue repair, collagen synthesis, and overall skin revitalization. The addition of Hyaluronic Acid, known for its exceptional moisture retention properties, enhances skin hydration, plumping, and elasticity. The PRP+HA Tube is specifically created to synergistically address fine lines, wrinkles, uneven skin texture, and early hair loss. Its innovative composition promotes natural-looking outcomes while offering the potential for improved skin radiance, hair density, and patient satisfaction. It’s important to note that the use of the PRP+HA Tube requires professional expertise to ensure safe and personalized treatment administration, aligning with the product’s intended purpose of delivering advanced and effective aesthetic solutions.
NOTE:
• For Single Patient Use Only. Once used, the blood collection tube must be discarded following the protocol for biohazard waste disposal, as required by the facility of use.
• The blood collection tube must be processed via centrifuge within 6 (six) minutes following blood draw.
• The PRP must be used within 4 (four) hours after drawing blood.
STORAGE
Store tubes at 4-40°C (39-104°F). Do not freeze.
CAUTION
a) PRP must be used on the same patient from which blood was drawn.
b) Do not use blood collection tube if any foreign matter is visible.
c) Do not use blood collection tubes that are, or appear to be, cracked or damaged.
d) Do not use blood collection tube after expiration date.
e) Do not re-sterilize or attempt to clean the blood collection tubes.
f) The PRP must be used within 4 (four) hours after drawing blood.
g) Practice Universal Precautions to protect from blood spatter and blood leak age to minimize the potential exposure to blood borne pathogens.
h) Handle all biologic samples and blood collection devices per the procedures established by facility of use.
i) Appropriate medical attention is required in the event of any exposure to biologic samples.
j) Under-filled tubes may result in poor product performance. It is recommended to discard an under-filled tube and re-draw blood into a second tube.